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The collection is focused on understanding how attitudes and representations of transgender people have developed and changed over time. It looks at three broad aspects of transgender: biology and the body, gender expression, and erotic expression and representation through the lenses of expert knowledge, as recorded by scientists and social scientists; transgender-community-member knowledge, as recorded by and for transgender people themselves; and common-sense knowledge, as recorded by and for members of the general public.

This extensive collection includes transgender books, magazines, newspaper clippings, scholarly papers, audio tapes, video tapes, films, photographs, artifacts, ephemera, biographical and autobiographical writings, and miscellaneous papers.